At least I now know how the quadrupole works. 0000049036 00000 n 0000008192 00000 n An athlete takes a performance-enhancing drug such as methenolone (brand name Primobolan). 124 54 0000010158 00000 n trailer 0000011142 00000 n 0000023088 00000 n Now you know has a mass spectrometer works – just remember the four stages in order – ionisation, acceleration, deflection, and detection. 0000004718 00000 n In a nutshell, mass spectrometry accurately measures the mass of different molecules within a sample. 0000002628 00000 n 0000003162 00000 n 0000004073 00000 n We also share information about your use of our site with our social media, advertising and analytics partners who may combine it with other information that you’ve provided to them or that they’ve collected from your use of their services. 0000010122 00000 n Organic chemists can use a mass spectrometer to ionize (or ‘smash’) a molecular compound in gaseous form, sort the fragments, and then identify the molecule fragments based on their molecular weights. 124 0 obj <> endobj Suppose you had a cannonball traveling past you and you wanted to deflect it as it went by you. 0000049077 00000 n The drug is either injected or taken orally. 0000011104 00000 n 0000048952 00000 n 0000006653 00000 n %PDF-1.6 %���� xref 0000049099 00000 n The lab informs the league of positive results. 0000009004 00000 n 0000002455 00000 n It tests the urine for the presence of the steroid or chemicals that are produced as the body processes the steroid, which may be detected for days to weeks after the last dose, depending on the drug and the amount taken. The instrument is an analytical tool known as a. 0000003420 00000 n 0000008739 00000 n There are many variations on the mass spectrometry theme, but the fundamentals are the same and are approximately three (it can depend on how you prefer to “group” your steps) and they are: Ionization (ion source) Ion Separation/Isolation (mass … 0000049144 00000 n ���fD����/E�I��wu��-����� �1 �-��/��D Company Number 11133744, How much of each of the components is present. 0000048909 00000 n During a random drug test, the athlete is asked to donate some of his urine to science. 0000002790 00000 n To do this, a high energy beam of electrons is passed through the sample molecule to produce electrically charged particles or ions. 0000048785 00000 n The technique is useful for identifying and quantifying the compounds in a mixture, which can include detecting impurities in a sample or determining the mixture of proteins in a cell sample. 0000002959 00000 n 0000003198 00000 n 0000012709 00000 n 0000012824 00000 n 0000004510 00000 n The MS will break each separated compound coming from the GC into ionised fragments. The instrument is an analytical tool known as a mass spectrometer. Chemists in the facility introduce a sample of the urine into an instrument that looks like three photocopiers connected together. The instrument used in MS is called mass spectrometer. @;�>)�_E��5�s�)� ���˃��N�ǥ��:��J��C�����>�_>S�t�?_�� �� The substance is bombarded with a beam of electrons so the atoms or molecules it contains are turned into ions. How does mass spectrometry work? There are many different kinds of mass spectrometers, but all use magnetic and/or electric fields to exert forces on the charged particles produced from the chemicals to be analyzed. 0000002241 00000 n Summary. 0000013550 00000 n The most common data representation is the mass spectrum. 0000001407 00000 n A mass spectrum is generated, which shows the different m/z values of ions present, and their relative abundance. You place the substance you want to study in a vacuum chamber inside the machine. ­­­­­­­C­reating and accelerating ions are essentially preparatory steps to the real work of mass spectrometry -- mass analysis. We use cookies to personalise content and ads, to provide social media features and to analyse our traffic. 0000002043 00000 n